Adamson Lab Members; *co-first or co-corresponding
Adamson lab publications and preprints
Cirincione A*, Simpson D*, Yan W, McNulty R, Ravisankar P, Solley SC, Yan J, Lim F, Farley EK, Singh M, Adamson B. A benchmarked, high-efficiency prime editing platform for multiplexed dropout screening. Nature Methods (2024).
McNamara H, Solley SC, Adamson B, Chan MM, Toettcher JE. Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking. Nature Cell Biology (2024).
Nguyen LT, Rakestraw NR, Pizzano BLM, Young CB, Huang Y, Beerensson KT, Antal SG, Anamisis KV, Peggs CMD, Yan J, Jing Y, Burdine RD, Adamson B, Toettcher JE, Myrvold C, Jain PK. Efficient Genome Editing with Chimeric Oligonucleotide-Directed Editing. bioRxiv (2024).
Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu J-C, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR. Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes. bioRxiv (2024).
Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parson LR, Adamson B. Improving prime editing with an endogenous small RNA-binding protein. Nature (2024).
Kim-Yip, RP*, McNulty R*, Joyce B, Mollica A, Chen PJ, Ravisankar P, Law BK, Liu DR, Toettcher JE, Ivakine EA, Posfai E, Adamson B. Efficient prime editing in two-cell mouse embryos using PEmbryo. Nature Biotechnology (2024).
Mesev EV, Lin AE, Guare EG, Heller BL, Douam F, Adamson B, Toettcher JE, Ploss A. Membrane-proximal motifs encode differences in signaling strength between type I and III interferon receptors. Science Signaling (2023).
Simpson D*, Ling J *, Jing Y, Adamson B. Mapping the Genetic Interaction Network of PARP inhibitor Response. bioRxiv (2023)
Wang B, Lin AE, Yuan J, Koch MD, Wingreen NS*, Adamson B*, Gitai Z*. Single-cell massively-parallel multiplexed microbial sequencing (M3-seq) identifies rare bacterial populations and profiles phage infection. Nature Microbiology (2023).
Hussmann JA, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, Simpson D, Yang D, Bothmer A, Cotta-Ramusino C*, Weissman JS*, Adamson B*. Mapping the Genetic Landscape of DNA Double-strand Break Repair. Cell 184, 5653-5669 (2021).
Chen PJ*, Hussmann JA*, Yan J,Knipping, F, Ravisankar P, Chen PF, Chen C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B*, Liu DR*. Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell 184, 5635-5652 (2021).
Koblan LW*, Arbab M*, Shen MW*, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B*, Liu DR*. Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology 39, 1414-1425 (2021).
Replogle JM., Norman TM., Xu A, Hussmann JA, Chen J, Cogan JZ, Meer EJ, Terry JM, Riordan DP, Srinivas N, Fiddes IT, Arthur JG, Alvarado LJ, Pfeiffer KA, Mikkelsen TS, Weissman JS*, & Adamson B*. Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing. Nature Biotechnology 38, 954-961 (2020).
Yan J, Cirincione A, & Adamson B. Prime Editing: Precision Genome Editing by Reverse Transcription. Molecular Cell 77, 110-112 (2020).
Britt's publications and preprints as a postdoc
Chen J, Brunner AD, Cogan JZ, Nuñez JK, Fields AP, Adamson B, Itzhak DN, Li JY, Mann M, Leonetti MD, & Weissman JS. Pervasive functional translation of noncanonical human open reading frames. Science 367, 1140-1146 (2020).
Chan MM*, Smith ZD*, Grosswendt S, Kretzmer H, Norman TM, Adamson B, Jost M, Quinn JJ, Yang D, Jones MG, Khodaverdian A, Yosef N, Meissner A*, & Weissman JS*. Molecular recording of mammalian embryogenesis. Nature 570, 77-82 (2019).
Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS*, & Gilbert LA*. Mapping the genetic landscape of human cells. Cell 174, 953-967 (2018).
Adamson B, Norman TM, Jost M, & Weissman JS. Approaches to maximize sgRNA-barcode coupling in Perturb-seq screens. bioRxiv (2018). Preprint never intended for publication.
Adamson B*, Norman TM*, Jost M, Cho MY, Nuñez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, & Weissman JS. A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response. Cell 167, 1867-1822 (2016).
Dixit A*, Parnas O*, Li B, Chen J, Fulco CP, Jerby-Arnon L, Marjanovic ND, Dionne D, Burks T, Raychowdhury R, Adamson B, Norman TM, Lander ES, Weissman JS, Friedman N, & Regev A. Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens. Cell 167, 1853-1866 (2016).
Horlbeck MA*, Gilbert LA*, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M*, & Weissman JS*. Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. eLife 5 (2016).
Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, & Weissman JS. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159, 647-661 (2014).
Britt's publications as a graduate and undergraduate student
Izhar L, Adamson B, Ciccia A, Lewis J, Pontano-Vaites L, Leng Y, Liang AC, Westbrook T, Harper JW, & Elledge SJ. A systematic analysis of factors localized to damaged chromatin reveals PARP-dependent recruitment of transcription factors. Cell Rep 11, 1486-1500 (2015).
Ciccia A, Nimonkar AV, Hu Y, Hajdu I, Achar YJ, Izhar L, Petit SA, Adamson B, Yoon JC, Kowalczykowski SC, Livingston DM, Haracska L, & Elledge SJ. Polyubiquitinated PCNA Recruits the ZRANB3 Translocase to Maintain Genomic Integrity after Replication Stress. Mol Cell 47, 396-409 (2012).
Adamson B, Smogorzewska A, Sigoillot FD, King RW, & Elledge SJ. A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response. Nat Cell Biol 14, 318-328 (2012).
Sigoillot FD, Lyman S, Huckins JF, Adamson B, Chung E, Quattrochi B, & King RW. A bioinformatics method identifies prominent off-targeted transcripts in RNAi screens. Nat Methods 9, 363-366 (2012).
O’Connell BC, Adamson B, Lydeard JR, Sowa ME, Ciccia A, Bredemeyer AL, Schlabach M, Gygi SP, Elledge SJ, & Harper JW. A Genome-wide Camptothecin Sensitivity Screen Identifies a Mammalian MMS22L-NFKBIL2 Complex Required for Genomic Stability. Mol Cell 40, 645-657 (2010).
Chou DM, Adamson B, Dephoure NE, Tan X, Nottke AC, Hurov KE, Gygi SP, Colaiácovo MP, & Elledge SJ. A chromatin localization screen reveals poly(ADP ribose)-regulated recruitment of the repressive polycomb and NuRD complexes to sites of DNA damage. Proc Natl Acad Sci USA 107, 18475-18480 (2010).